mouse spermatogenesis pathway cell line gc 2 spd ts Search Results


gc 2  (ATCC)
94
ATCC gc 2
Gc 2, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gc2  (Proteintech)
93
Proteintech gc2
( A ) Immunoblotting analysis of the expression of phototransduction proteins, including RHO, GC1, <t>GC2,</t> GRK1, and PDE6B in retinas of P30 WT and KO mice. GAPDH was used as a loading control. ( B ) Quantification of immunoblotting signals shown in ( A ). Data are expressed as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; unpaired t -test. ( C, D ) Immunostaining of P30 mouse retinal sections to label RHO, GC1, GC2, PDE6B, GRK1, and GCAP2 (green). Nuclei were label with DAPI (blue). OS, outer segment; IS, inner segment; ONL, outer nuclear layer. Scale bar, 20 μm. ( E ) COS-7 cells transfected to co-express recombinant RHO-mCherry with EGFP (upper panel) or REEP6-EGFP (lower panel). Nuclei were stained with DAPI (blue). Scale bar, 10 μm.
Gc2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+spermatogenesis+pathway+cell+line+gc+2+spd+ts/PDE6B+Antibody/bio_rxiv__2025__03__02__641069-73-23-38
Average 93 stars, based on 1 article reviews
gc2 - by Bioz Stars, 2026-10
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mouse pachytene spermatocyte derived cell line gc 2  (Procell Inc)
86
Procell Inc mouse pachytene spermatocyte derived cell line gc 2
( A ) Immunoblotting analysis of the expression of phototransduction proteins, including RHO, GC1, <t>GC2,</t> GRK1, and PDE6B in retinas of P30 WT and KO mice. GAPDH was used as a loading control. ( B ) Quantification of immunoblotting signals shown in ( A ). Data are expressed as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; unpaired t -test. ( C, D ) Immunostaining of P30 mouse retinal sections to label RHO, GC1, GC2, PDE6B, GRK1, and GCAP2 (green). Nuclei were label with DAPI (blue). OS, outer segment; IS, inner segment; ONL, outer nuclear layer. Scale bar, 20 μm. ( E ) COS-7 cells transfected to co-express recombinant RHO-mCherry with EGFP (upper panel) or REEP6-EGFP (lower panel). Nuclei were stained with DAPI (blue). Scale bar, 10 μm.
Mouse Pachytene Spermatocyte Derived Cell Line Gc 2, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+spermatogenesis+pathway+cell+line+gc+2+spd+ts/cell+line+mouse+spermatogonia/pmc13159132-305-7-16
Average 86 stars, based on 1 article reviews
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gc 2 spd mouse spermatocytes  (ATCC)
94
ATCC gc 2 spd mouse spermatocytes
( A ) Immunoblotting analysis of the expression of phototransduction proteins, including RHO, GC1, <t>GC2,</t> GRK1, and PDE6B in retinas of P30 WT and KO mice. GAPDH was used as a loading control. ( B ) Quantification of immunoblotting signals shown in ( A ). Data are expressed as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; unpaired t -test. ( C, D ) Immunostaining of P30 mouse retinal sections to label RHO, GC1, GC2, PDE6B, GRK1, and GCAP2 (green). Nuclei were label with DAPI (blue). OS, outer segment; IS, inner segment; ONL, outer nuclear layer. Scale bar, 20 μm. ( E ) COS-7 cells transfected to co-express recombinant RHO-mCherry with EGFP (upper panel) or REEP6-EGFP (lower panel). Nuclei were stained with DAPI (blue). Scale bar, 10 μm.
Gc 2 Spd Mouse Spermatocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+spermatogenesis+pathway+cell+line+gc+2+spd+ts/GC-2spd(ts)%3B+Spermatocyte%3B+Mouse/pmc09947114-48-6-10
Average 94 stars, based on 1 article reviews
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mouse spermatocyte derived gc 2 cells  (ATCC)
95
ATCC mouse spermatocyte derived gc 2 cells
( A ) Immunoblotting analysis of the expression of phototransduction proteins, including RHO, GC1, <t>GC2,</t> GRK1, and PDE6B in retinas of P30 WT and KO mice. GAPDH was used as a loading control. ( B ) Quantification of immunoblotting signals shown in ( A ). Data are expressed as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; unpaired t -test. ( C, D ) Immunostaining of P30 mouse retinal sections to label RHO, GC1, GC2, PDE6B, GRK1, and GCAP2 (green). Nuclei were label with DAPI (blue). OS, outer segment; IS, inner segment; ONL, outer nuclear layer. Scale bar, 20 μm. ( E ) COS-7 cells transfected to co-express recombinant RHO-mCherry with EGFP (upper panel) or REEP6-EGFP (lower panel). Nuclei were stained with DAPI (blue). Scale bar, 10 μm.
Mouse Spermatocyte Derived Gc 2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+spermatogenesis+pathway+cell+line+gc+2+spd+ts/GC-2spd(ts)/pm39109527-52-0-10
Average 95 stars, based on 1 article reviews
mouse spermatocyte derived gc 2 cells - by Bioz Stars, 2026-10
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gc 2 donkey anti rabbit igg alexa fluor 680 thermofisher a10043 polyclonal  (Biotium)
95
Biotium gc 2 donkey anti rabbit igg alexa fluor 680 thermofisher a10043 polyclonal
( A ) Immunoblotting analysis of the expression of phototransduction proteins, including RHO, GC1, <t>GC2,</t> GRK1, and PDE6B in retinas of P30 WT and KO mice. GAPDH was used as a loading control. ( B ) Quantification of immunoblotting signals shown in ( A ). Data are expressed as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; unpaired t -test. ( C, D ) Immunostaining of P30 mouse retinal sections to label RHO, GC1, GC2, PDE6B, GRK1, and GCAP2 (green). Nuclei were label with DAPI (blue). OS, outer segment; IS, inner segment; ONL, outer nuclear layer. Scale bar, 20 μm. ( E ) COS-7 cells transfected to co-express recombinant RHO-mCherry with EGFP (upper panel) or REEP6-EGFP (lower panel). Nuclei were stained with DAPI (blue). Scale bar, 10 μm.
Gc 2 Donkey Anti Rabbit Igg Alexa Fluor 680 Thermofisher A10043 Polyclonal, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
gc 2 donkey anti rabbit igg alexa fluor 680 thermofisher a10043 polyclonal - by Bioz Stars, 2026-10
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ggnbp2ko gc 2 cells ggnbp2 double nickase plasmids  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology ggnbp2ko gc 2 cells ggnbp2 double nickase plasmids
( A ) Immunoblotting analysis of the expression of phototransduction proteins, including RHO, GC1, <t>GC2,</t> GRK1, and PDE6B in retinas of P30 WT and KO mice. GAPDH was used as a loading control. ( B ) Quantification of immunoblotting signals shown in ( A ). Data are expressed as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; unpaired t -test. ( C, D ) Immunostaining of P30 mouse retinal sections to label RHO, GC1, GC2, PDE6B, GRK1, and GCAP2 (green). Nuclei were label with DAPI (blue). OS, outer segment; IS, inner segment; ONL, outer nuclear layer. Scale bar, 20 μm. ( E ) COS-7 cells transfected to co-express recombinant RHO-mCherry with EGFP (upper panel) or REEP6-EGFP (lower panel). Nuclei were stained with DAPI (blue). Scale bar, 10 μm.
Ggnbp2ko Gc 2 Cells Ggnbp2 Double Nickase Plasmids, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+spermatogenesis+pathway+cell+line+gc+2+spd+ts/Ggnbp2+CRISPR%2FCas9+KO+Plasmid/pm39109527-54-3-16
Average 92 stars, based on 1 article reviews
ggnbp2ko gc 2 cells ggnbp2 double nickase plasmids - by Bioz Stars, 2026-10
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gc 2 spd cells  (ATCC)
95
ATCC gc 2 spd cells
( A ) Immunoblotting analysis of the expression of phototransduction proteins, including RHO, GC1, <t>GC2,</t> GRK1, and PDE6B in retinas of P30 WT and KO mice. GAPDH was used as a loading control. ( B ) Quantification of immunoblotting signals shown in ( A ). Data are expressed as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; unpaired t -test. ( C, D ) Immunostaining of P30 mouse retinal sections to label RHO, GC1, GC2, PDE6B, GRK1, and GCAP2 (green). Nuclei were label with DAPI (blue). OS, outer segment; IS, inner segment; ONL, outer nuclear layer. Scale bar, 20 μm. ( E ) COS-7 cells transfected to co-express recombinant RHO-mCherry with EGFP (upper panel) or REEP6-EGFP (lower panel). Nuclei were stained with DAPI (blue). Scale bar, 10 μm.
Gc 2 Spd Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+spermatogenesis+pathway+cell+line+gc+2+spd+ts/GC-1+spg/pm25591775-48-5-16
Average 95 stars, based on 1 article reviews
gc 2 spd cells - by Bioz Stars, 2026-10
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human gc cdna probe  (ATCC)
93
ATCC human gc cdna probe
Expression of GC mRNA and enzyme in RecNciI and L444P mice. (A) A Northern blot with 5 μg of brain poly(A)+ RNA from a RecNciI homozygote mouse, a L444P homozygote mouse, a L444P heterozygote mouse, and a wild-type mouse. The blot first was probed with a mouse <t>GC</t> <t>cDNA.</t> After stripping, it was reprobed with a mouse metaxin cDNA. Finally, it was stripped once more and probed with a human actin cDNA. (B) Acid β-glucosidase activity was determined in extracts of liver, brain, and skin from homozygote L444P and RecNciI mice. Tissues from three mice of each genotype were assayed. The assays were repeated three times. The activity (±SD) is expressed as nmol substrate cleaved/hr per mg of protein.
Human Gc Cdna Probe, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+spermatogenesis+pathway+cell+line+gc+2+spd+ts/GC-2%2E2+(GCS-2KB)+PLASMID+IN+ESCHERICHIA+COLI/pmc00019391-42-20-24
Average 93 stars, based on 1 article reviews
human gc cdna probe - by Bioz Stars, 2026-10
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murine spermatocyte derived cell line  (ATCC)
96
ATCC murine spermatocyte derived cell line
Expression of GC mRNA and enzyme in RecNciI and L444P mice. (A) A Northern blot with 5 μg of brain poly(A)+ RNA from a RecNciI homozygote mouse, a L444P homozygote mouse, a L444P heterozygote mouse, and a wild-type mouse. The blot first was probed with a mouse <t>GC</t> <t>cDNA.</t> After stripping, it was reprobed with a mouse metaxin cDNA. Finally, it was stripped once more and probed with a human actin cDNA. (B) Acid β-glucosidase activity was determined in extracts of liver, brain, and skin from homozygote L444P and RecNciI mice. Tissues from three mice of each genotype were assayed. The assays were repeated three times. The activity (±SD) is expressed as nmol substrate cleaved/hr per mg of protein.
Murine Spermatocyte Derived Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+spermatogenesis+pathway+cell+line+gc+2+spd+ts/TM4/pm39709656-164-1-17
Average 96 stars, based on 1 article reviews
murine spermatocyte derived cell line - by Bioz Stars, 2026-10
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anti gao  (Bio-Rad)
85
Bio-Rad anti gao
Expression of GC mRNA and enzyme in RecNciI and L444P mice. (A) A Northern blot with 5 μg of brain poly(A)+ RNA from a RecNciI homozygote mouse, a L444P homozygote mouse, a L444P heterozygote mouse, and a wild-type mouse. The blot first was probed with a mouse <t>GC</t> <t>cDNA.</t> After stripping, it was reprobed with a mouse metaxin cDNA. Finally, it was stripped once more and probed with a human actin cDNA. (B) Acid β-glucosidase activity was determined in extracts of liver, brain, and skin from homozygote L444P and RecNciI mice. Tissues from three mice of each genotype were assayed. The assays were repeated three times. The activity (±SD) is expressed as nmol substrate cleaved/hr per mg of protein.
Anti Gao, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+spermatogenesis+pathway+cell+line+gc+2+spd+ts/Unity+Real+Time+Installation+Package/pm17126529-68-32-12
Average 85 stars, based on 1 article reviews
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mgc27016 antibody - n-terminal region (arp41134_p050)  (Aviva Systems)
N/A
This is a rabbit polyclonal antibody against MGC27016. It was validated on Western Blot and immunohistochemistry by Aviva Systems Biology. At Aviva Systems Biology we manufacture rabbit polyclonal antibodies on a large scale (200-1000 products/month)
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Image Search Results


( A ) Immunoblotting analysis of the expression of phototransduction proteins, including RHO, GC1, GC2, GRK1, and PDE6B in retinas of P30 WT and KO mice. GAPDH was used as a loading control. ( B ) Quantification of immunoblotting signals shown in ( A ). Data are expressed as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; unpaired t -test. ( C, D ) Immunostaining of P30 mouse retinal sections to label RHO, GC1, GC2, PDE6B, GRK1, and GCAP2 (green). Nuclei were label with DAPI (blue). OS, outer segment; IS, inner segment; ONL, outer nuclear layer. Scale bar, 20 μm. ( E ) COS-7 cells transfected to co-express recombinant RHO-mCherry with EGFP (upper panel) or REEP6-EGFP (lower panel). Nuclei were stained with DAPI (blue). Scale bar, 10 μm.

Journal: bioRxiv

Article Title: REEP6 deficiency impairs ER and Golgi morphologies and causes retinal degeneration by attenuating the expression of phototransduction proteins

doi: 10.1101/2025.03.02.641069

Figure Lengend Snippet: ( A ) Immunoblotting analysis of the expression of phototransduction proteins, including RHO, GC1, GC2, GRK1, and PDE6B in retinas of P30 WT and KO mice. GAPDH was used as a loading control. ( B ) Quantification of immunoblotting signals shown in ( A ). Data are expressed as mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; unpaired t -test. ( C, D ) Immunostaining of P30 mouse retinal sections to label RHO, GC1, GC2, PDE6B, GRK1, and GCAP2 (green). Nuclei were label with DAPI (blue). OS, outer segment; IS, inner segment; ONL, outer nuclear layer. Scale bar, 20 μm. ( E ) COS-7 cells transfected to co-express recombinant RHO-mCherry with EGFP (upper panel) or REEP6-EGFP (lower panel). Nuclei were stained with DAPI (blue). Scale bar, 10 μm.

Article Snippet: The sections were immunostained as previously described using primary antibodies against the following proteins: rhodopsin (1:1,000, custom-made ( )), GC1 (1:200, Proteintech, China), GC2 (1:500, a gift from Dr. Wolfgang Baehr at the University of Utah), PDE6B (1:500, Proteintech, China), GRK1 (1:200, Proteintech, China), and GCAP2 (1:500, a gift from Dr. Wolfgang Baehr at the University of Utah).

Techniques: Western Blot, Expressing, Control, Immunostaining, Transfection, Recombinant, Staining

Expression of GC mRNA and enzyme in RecNciI and L444P mice. (A) A Northern blot with 5 μg of brain poly(A)+ RNA from a RecNciI homozygote mouse, a L444P homozygote mouse, a L444P heterozygote mouse, and a wild-type mouse. The blot first was probed with a mouse GC cDNA. After stripping, it was reprobed with a mouse metaxin cDNA. Finally, it was stripped once more and probed with a human actin cDNA. (B) Acid β-glucosidase activity was determined in extracts of liver, brain, and skin from homozygote L444P and RecNciI mice. Tissues from three mice of each genotype were assayed. The assays were repeated three times. The activity (±SD) is expressed as nmol substrate cleaved/hr per mg of protein.

Journal:

Article Title: Mice with type 2 and 3 Gaucher disease point mutations generated by a single insertion mutagenesis procedure (SIMP)

doi:

Figure Lengend Snippet: Expression of GC mRNA and enzyme in RecNciI and L444P mice. (A) A Northern blot with 5 μg of brain poly(A)+ RNA from a RecNciI homozygote mouse, a L444P homozygote mouse, a L444P heterozygote mouse, and a wild-type mouse. The blot first was probed with a mouse GC cDNA. After stripping, it was reprobed with a mouse metaxin cDNA. Finally, it was stripped once more and probed with a human actin cDNA. (B) Acid β-glucosidase activity was determined in extracts of liver, brain, and skin from homozygote L444P and RecNciI mice. Tissues from three mice of each genotype were assayed. The assays were repeated three times. The activity (±SD) is expressed as nmol substrate cleaved/hr per mg of protein.

Article Snippet: Genomic clones from a 129/sv strain library (Stratagene; catalogue 946306) containing the mouse GC gene were isolated by using a human GC cDNA probe (ATCC; catalogue 65696).

Techniques: Expressing, Northern Blot, Stripping Membranes, Activity Assay